Tim Lei is an assistant professor in the Department of Electrical Engineering at the Downtown Campus of the University of Colorado Denver. He also holds adjunct faculty positions in the Departments of Bioengineering, Physiology & Biophysics and Medicine at the Anschutz Medical Campus. Tim is an expert in advanced fluorescence microscopy techniques, multiphoton microscopy and spectroscopic techniques in biological systems. He currently serves as a technical advisor of the Colorado Advanced Photonic Technology (CAPT) Laboratory at the College of Engineering and Applied Sciences. He is also a member of the steering committee of the Advanced Light Microscopy Core (ALMC) at the Anschutz Medical Campus.
Tim's research interest is to apply advanced fluorescence microscopy and linear and nonlinear spectroscopy techniques in biomedical applications. Tim is currently working on using these techniques to understand phosphate imbalance in chronic kidney disease, progression and treatment of glaucoma in the eye, developing optical and electronic devices to understand Parkinson's disease with optogenetic protein technology, and study the nonlinear optical response of next generation photodynamic therapy drugs.
On the teaching side, Tim developed several courses in optics and biophotonics in the graduate level to allow students to engage in advanced biophotonic research. He also teaches several undergraduate level courses in electrical engineering including circuit design and microelectronics.
- Protein-protein interactions in live cells using fluorescence correlational techniques, such as fluorescence correlation spectroscopy (FCS).
- Multiphoton microscopy imaging including second harmonic generation (SHG), two-photon autofluorescence (TPAF) and coherent anti-Stokes Raman spectroscopy (CARS).
- Device development for optogenetic protein technology to study neural circuits in animal models and future human disease treatments.
- Charaterization of nonlinear optical properties of next generation photodynamic therapy photosensitizers.